Coding

Part:BBa_K2121009:Design

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


Rhamnose_Lambda_Beta_A6UAS9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 777
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Expression fusion with native S. meliloti protein.


Source

Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.

References